Reference Lab Services

Targeted Sequencing

Kashi Clinical Laboratories can give breadth and flexibility to your genetic analysis using targeted sequencing technologies with the Ion PGM or Proton System. Targeted sequencing enables scientists to pinpoint and magnify specific regions within the human genome. Such targeted analysis is ideal for clinical research by increasing cost-effectiveness, throughput, and scalability.

There are a number of applications for targeted sequencing, including the detection of various genetic aberrations such as single-nucleotide polymorphisms (SNP's), insertions and deletions (indels), copy number variants (CNV's), and genetic rearrangements. Some applications include cancer genomics, cancer mutation analysis, and genetic disease/disorder research - including analysis of FFPE (formalin-fixed paraffin-embedded) tissue samples.

Ion AmpliSeq ready-to-use targeted primer panels, or custom-designed panels, contain primer pairs that are specifically designed to flank regions of interest for polymerase chain reaction (PCR) amplification with as low as 10 ng of input DNA.

Targeted Sequencing Panels Offered by Kashi Genomics

  • The most comprehensive cancer panel available, the Ion AmpliSeq Comprehensive Cancer Panel provides complete coverage of over 400 of the genes most frequently implicated in cancer including KRAS, EGFR, FLT3, PTEN, TP53 and many others.
  • For a more focused and cost effective approach to identify cancer variants the Ion Ampliseq Cancer Hotspot Panel v2 targets approximately 2,800 COSMIC mutations from 50 oncogenes and tumor suppressor genes.
  • The Ion Ampliseq Inherited Disease Panel provides coverage of the coding regions of 300 genes that are implicated in over 700 inherited Mendelian diseases.
  • To target specific genomic regions of interest, the Kashi Genomics team is poised to develop Custom-design Ampliseq Panels to provide a cost-effective laboratory solution that is tailored to your specific project.